Methods for Cross‐Linking Nucleic Acids

NMR methods for studying quadruplex nucleic acids.

Solution NMR spectroscopy has traditionally played a central role in examining quadruplex structure, dynamics, and interactions. Here, an overview is given of the methods currently applied to structural, dynamics, thermodynamics, and kinetics studies of nucleic acid quadruplexes and associated cations.

New methods for probing nucleic acids.

Modern molecular biology depends on the ability to manipulate DNA. Cloning and other genetic engineering techniques require the specific cleavage of DNA at restriction sites (see below). One method of sequencing nucleic acids relies on base-specific chemical reactions, and several structure-specific ribonucleases are used to probe the secondary and tertiary structure of RNA. There is a great de...

Methods for Extracting Nucleic Acids from Mars Analog Regolith

New methods for the quantitation of microbial nucleic acids

Nucleic acid amplification reactions offer a great deal of potential for the accurate and sensitive detectionlquantitation of microbial nucleic acids in human body fluids and have, in some cases, an edge over techniques using immunoassays. Successful detections include : nucleic acids from viruses such as HIV, rotaviruses, coxsackieviruses, papilloma viruses, pestiviruses as well as microorgani...

Nonisotopic detection methods for strand displacement assays of nucleic acids.

Using the enzymes terminal deoxyribonucleotidyltransferase (EC 2.7.7.31) and polynucleotide phosphorylase (EC 2.7.7.8), we constructed polyriboadenylic acid tracts, approximately 8000 AMP residues long, attached to the 3'-terminus of a synthetic deoxynucleotide. The polyadenylated DNA, termed the "signal strand", was used in a displacement-type nucleic acid probe assay (see pp 1631-6, this issu...